2024
Celik, Asena Aslihan; Ayhan, Kamuran; Tok, Kenan Can; Gumustas, Mehmet; Altuntas, Evrim Gunes
Lactic acid bacteria from fermented products: Antimicrobial activity and metabolite production Journal Article
In: Latin American Applied Research, vol. 54, no. 4, pp. 585-593, 2024, ISSN: 1851-8796.
@article{Celik2024,
title = {Lactic acid bacteria from fermented products: Antimicrobial activity and metabolite production},
author = {Asena Aslihan Celik and Kamuran Ayhan and Kenan Can Tok and Mehmet Gumustas and Evrim Gunes Altuntas},
url = {https://laar.plapiqui.edu.ar/OJS/index.php/laar/article/view/3413},
doi = {10.52292/j.laar.2024.3413},
issn = {1851-8796},
year = {2024},
date = {2024-10-07},
urldate = {2024-10-07},
journal = {Latin American Applied Research},
volume = {54},
number = {4},
pages = {585-593},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Altuntas, Evrim Gunes; Celık, Asena Aslihan; Sevım, Busra; Tok, Kenan Can; Gumustas, Mehmet; Mergen, Hatice; Juneja, Vijay K
A Novel Bioprotective Strain of F2-Physiological, Genetical, and Antimicrobial Characterization Journal Article
In: Foodborne Pathog Dis, 2024, ISSN: 1556-7125.
@article{pmid39258750,
title = {A Novel Bioprotective Strain of F2-Physiological, Genetical, and Antimicrobial Characterization},
author = {Evrim Gunes Altuntas and Asena Aslihan Celık and Busra Sevım and Kenan Can Tok and Mehmet Gumustas and Hatice Mergen and Vijay K Juneja},
doi = {10.1089/fpd.2024.0005},
issn = {1556-7125},
year = {2024},
date = {2024-09-01},
urldate = {2024-09-01},
journal = {Foodborne Pathog Dis},
abstract = { is a member of lactic acid bacteria that improves the quality of fermented foods while also having a positive impact on human health. In this study, F2 was studied for characteristics such as biochemical and genetic identification, metabolite production, antimicrobial activity, and plasmid content. This strain exerts antimicrobial activity against some Gram-positive and Gram-negative pathogens (, , , and ) with inhibition zone diameters ranging between 17.0 and 29.0 mm; it can ferment glucose, arabinose, galactose, lactose, and demonstrated the ability to grow at high temperature (50°C). Another physiological specification of the strain was the morphology of the isolate in selective medium, the de Man, Rogosa, Sharpe medium (MRS medium containing triphenyl tetrazolium chloride), which exhibits a chromogenic colony (characterized as purple colonies) on the modified-MRS (mMRS) medium. Metabolites such as lactic acid and diacetyl production of the strain F2 were also investigated using chromatography and found to be 10.07 and 0.05 µg/mL, respectively. The peptides of the isolate's cell-free supernatant were determined to be ∼80 kDa, and finally, the plasmid isolated from the strain F2 was identified as strain KLDS1.0386 plasmid p4, which may be responsible for some characteristic properties, such as antimicrobial peptide production of the strain.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
is a member of lactic acid bacteria that improves the quality of fermented foods while also having a positive impact on human health. In this study, F2 was studied for characteristics such as biochemical and genetic identification, metabolite production, antimicrobial activity, and plasmid content. This strain exerts antimicrobial activity against some Gram-positive and Gram-negative pathogens (, , , and ) with inhibition zone diameters ranging between 17.0 and 29.0 mm; it can ferment glucose, arabinose, galactose, lactose, and demonstrated the ability to grow at high temperature (50°C). Another physiological specification of the strain was the morphology of the isolate in selective medium, the de Man, Rogosa, Sharpe medium (MRS medium containing triphenyl tetrazolium chloride), which exhibits a chromogenic colony (characterized as purple colonies) on the modified-MRS (mMRS) medium. Metabolites such as lactic acid and diacetyl production of the strain F2 were also investigated using chromatography and found to be 10.07 and 0.05 µg/mL, respectively. The peptides of the isolate's cell-free supernatant were determined to be ∼80 kDa, and finally, the plasmid isolated from the strain F2 was identified as strain KLDS1.0386 plasmid p4, which may be responsible for some characteristic properties, such as antimicrobial peptide production of the strain.
Hurkul, M Mesud; Cetinkaya, Ahmet; Yayla, Seyda; Kaya, S Irem; Budak, Fatma; Tok, Kenan Can; Gumustas, Mehmet; Uzun, Lokman; Ozkan, Sibel A
In: Talanta, vol. 273, pp. 125883, 2024, ISSN: 1873-3573.
@article{pmid38521023,
title = {Highly selective and sensitive molecularly imprinted sensors for the electrochemical assay of quercetin in methanol extracts of Rubus sanctus and Fragaria vesca},
author = {M Mesud Hurkul and Ahmet Cetinkaya and Seyda Yayla and S Irem Kaya and Fatma Budak and Kenan Can Tok and Mehmet Gumustas and Lokman Uzun and Sibel A Ozkan},
doi = {10.1016/j.talanta.2024.125883},
issn = {1873-3573},
year = {2024},
date = {2024-03-01},
urldate = {2024-03-01},
journal = {Talanta},
volume = {273},
pages = {125883},
abstract = {Quercetin (QUE) is a powerful antioxidant and one of the common phenolic compounds found in plants, vegetables, and fruits, which has shown many pharmacological activities. The complex nature of the matrix in which QUE is found and its importance and potential uses in diverse applications force the researchers to develop selective and sensitive sensors. In the present work, a novel molecularly imprinted polymer (MIP)-based electrochemical sensor was fabricated for the selective and sensitive determination of the QUE in plant extracts and food supplements. Tryptophan methacrylate (TrpMA) was chosen as the functional monomer, whereas the photopolymerization (PP) method was applied using a glassy carbon electrode (GCE). Electrochemical and morphological characterizations of the developed sensor (TrpMA@QUE/MIP-GCE) were performed using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and scanning electron microscopy (SEM). The linear range of the developed sensor was determined to be in the range of 1.0-25 pM, while the limit of detection (LOD) was calculated to be 0.235 pM. In conclusion, The TrpMA@QUE/MIP-GCE sensor might be classified as a promising platform for selective and sensitive determination of QUE not only in plant extracts but also in commercial food supplements because of its reliability, reproducibility, repeatability, stability, and fast response time.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Quercetin (QUE) is a powerful antioxidant and one of the common phenolic compounds found in plants, vegetables, and fruits, which has shown many pharmacological activities. The complex nature of the matrix in which QUE is found and its importance and potential uses in diverse applications force the researchers to develop selective and sensitive sensors. In the present work, a novel molecularly imprinted polymer (MIP)-based electrochemical sensor was fabricated for the selective and sensitive determination of the QUE in plant extracts and food supplements. Tryptophan methacrylate (TrpMA) was chosen as the functional monomer, whereas the photopolymerization (PP) method was applied using a glassy carbon electrode (GCE). Electrochemical and morphological characterizations of the developed sensor (TrpMA@QUE/MIP-GCE) were performed using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and scanning electron microscopy (SEM). The linear range of the developed sensor was determined to be in the range of 1.0-25 pM, while the limit of detection (LOD) was calculated to be 0.235 pM. In conclusion, The TrpMA@QUE/MIP-GCE sensor might be classified as a promising platform for selective and sensitive determination of QUE not only in plant extracts but also in commercial food supplements because of its reliability, reproducibility, repeatability, stability, and fast response time.
Demirbugen-Oz, Merve; Ozdemir, Fezile; Tok, Kenan Can; Dural, Emrah; Kir, Yagmur; Ulusoy, Muge; Gumustas, Mehmet; Baskak, Bora; Suzen, Halit Sinan
Assessment of Genetic and Lifestyle Factors on Clozapine and N-desmethylclozapine Plasma Levels in Schizophrenia Patients Journal Article
In: Pharmacopsychiatry, vol. 57, no. 02, pp. 87, 2024, ISSN: 1439-0795.
@article{Demirbugen2024,
title = {Assessment of Genetic and Lifestyle Factors on Clozapine and N-desmethylclozapine Plasma Levels in Schizophrenia Patients},
author = {Merve Demirbugen-Oz and Fezile Ozdemir and Kenan Can Tok and Emrah Dural and Yagmur Kir and Muge Ulusoy and Mehmet Gumustas and Bora Baskak and Halit Sinan Suzen},
doi = {10.1055/s-0044-1779559},
issn = {1439-0795},
year = {2024},
date = {2024-03-00},
urldate = {2024-03-00},
journal = {Pharmacopsychiatry},
volume = {57},
number = {02},
pages = {87},
publisher = {Georg Thieme Verlag},
abstract = {Plasma concentrations of clozapine (CLZ) vary due to several factors, including age, sex, smoking habits, caffeine consumption, and comedication. Genetic variations might also explain the variations for plasma CLZ (2). Among genetic polymorphisms that may have a role in CLZ metabolism, CYP1A2*1F is a strong candidate.. Cytochrome P450 oxidoreductase (POR) gene POR*28 variation is also potentially a considerable target.
Within this context, we have investigated the influence of genetic (CYP1A2*1F and POR*28) polymorphisms and non-genetic factors (caffeine consumption and cigarette smoking) on CLZ and main metabolite DCLZ concentrations. For this purpose, 112 schizophrenia patients receiving CLZ were included in the current study. Plasma CLZ and N-desmethylclozapine (DCLZ) levels were analysed by using HPLC-UV detector set at 220 nm. Genetic variations were identified using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay.
There was no significant difference between the genotype groups on CLZ, DCLZ levels, and DCLZ/CLZ ratios. In the subgroups created according to the patient’s CYP1A2 genotype, and smoking and caffeine consumption status, significant differences have been observed between POR*28 wild-type and variant genotype groups. Regression model analyses revealed that the POR*28 polymorphism correlates significantly with both CLZ and DLCZ (ng/ml/mg/kg) levels.
The outcome of our investigation findings suggests that, considering both genetic and non-genetic factors may have the potential to increase efficacy and decrease adverse drug reactions in the treatment of schizophrenia with CLZ.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Plasma concentrations of clozapine (CLZ) vary due to several factors, including age, sex, smoking habits, caffeine consumption, and comedication. Genetic variations might also explain the variations for plasma CLZ (2). Among genetic polymorphisms that may have a role in CLZ metabolism, CYP1A2*1F is a strong candidate.. Cytochrome P450 oxidoreductase (POR) gene POR*28 variation is also potentially a considerable target.
Within this context, we have investigated the influence of genetic (CYP1A2*1F and POR*28) polymorphisms and non-genetic factors (caffeine consumption and cigarette smoking) on CLZ and main metabolite DCLZ concentrations. For this purpose, 112 schizophrenia patients receiving CLZ were included in the current study. Plasma CLZ and N-desmethylclozapine (DCLZ) levels were analysed by using HPLC-UV detector set at 220 nm. Genetic variations were identified using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay.
There was no significant difference between the genotype groups on CLZ, DCLZ levels, and DCLZ/CLZ ratios. In the subgroups created according to the patient’s CYP1A2 genotype, and smoking and caffeine consumption status, significant differences have been observed between POR*28 wild-type and variant genotype groups. Regression model analyses revealed that the POR*28 polymorphism correlates significantly with both CLZ and DLCZ (ng/ml/mg/kg) levels.
The outcome of our investigation findings suggests that, considering both genetic and non-genetic factors may have the potential to increase efficacy and decrease adverse drug reactions in the treatment of schizophrenia with CLZ.
Within this context, we have investigated the influence of genetic (CYP1A2*1F and POR*28) polymorphisms and non-genetic factors (caffeine consumption and cigarette smoking) on CLZ and main metabolite DCLZ concentrations. For this purpose, 112 schizophrenia patients receiving CLZ were included in the current study. Plasma CLZ and N-desmethylclozapine (DCLZ) levels were analysed by using HPLC-UV detector set at 220 nm. Genetic variations were identified using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay.
There was no significant difference between the genotype groups on CLZ, DCLZ levels, and DCLZ/CLZ ratios. In the subgroups created according to the patient’s CYP1A2 genotype, and smoking and caffeine consumption status, significant differences have been observed between POR*28 wild-type and variant genotype groups. Regression model analyses revealed that the POR*28 polymorphism correlates significantly with both CLZ and DLCZ (ng/ml/mg/kg) levels.
The outcome of our investigation findings suggests that, considering both genetic and non-genetic factors may have the potential to increase efficacy and decrease adverse drug reactions in the treatment of schizophrenia with CLZ.
2023
Oz, Merve Demirbugen; Ozdemir, Fezile; Tok, Kenan Can; Dural, Emrah; Kir, Yagmur; Ulusoy, Muge; Gumustas, Mehmet; Baskak, Bora; Suzen, H. Sinan
In: Expert Opinion on Drug Metabolism & Toxicology, pp. 1–9, 2023, ISSN: 1744-7607.
@article{DemirbugenOz2023,
title = {The Potential Role of \textit{POR*28} and \textit{CYP1A2*F} Genetic Variations and Lifestyle Factors on Clozapine and N-DesmethylClozapine Plasma Levels in Schizophrenia Patients},
author = {Merve Demirbugen Oz and Fezile Ozdemir and Kenan Can Tok and Emrah Dural and Yagmur Kir and Muge Ulusoy and Mehmet Gumustas and Bora Baskak and H. Sinan Suzen},
doi = {10.1080/17425255.2023.2221849},
issn = {1744-7607},
year = {2023},
date = {2023-06-08},
urldate = {2023-06-08},
journal = {Expert Opinion on Drug Metabolism & Toxicology},
pages = {1--9},
publisher = {Informa UK Limited},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Tok, Kenan Can; Erbil, Gökhun Çağatay; Yayla, Şeyda; Kiymaci, Merve Eylül; Hurkul, Muhammed Mesud
Chemical Screening and Antibacterial Activity of Crude Extracts of Chlorella sp. in Culture Journal Article
In: Hacettepe University Journal of the Faculty of Pharmacy (HUJPHARM), 2023, ISSN: 2458-8806.
@article{TOK2023b,
title = {Chemical Screening and Antibacterial Activity of Crude Extracts of Chlorella sp. in Culture},
author = {Kenan Can Tok and Gökhun Çağatay Erbil and Şeyda Yayla and Merve Eylül Kiymaci and Muhammed Mesud Hurkul},
doi = {10.52794/hujpharm.1102486},
issn = {2458-8806},
year = {2023},
date = {2023-02-01},
urldate = {2023-02-01},
journal = {Hacettepe University Journal of the Faculty of Pharmacy (HUJPHARM)},
publisher = {Hacettepe University},
abstract = {<jats:p xml:lang="en">In this study, the antibacterial activity of methanol and acetone extracts of Chlorella sp. was examined. The chemical contents of the extracts were clarified by GC/MS analysis. Antibacterial activity of Chlorella sp. extracts was determined as a minimum inhibitory concentration by broth microdilution method against Bacillus subtilis ATCC 6633, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 29213. It was found that methanol and acetone extracts of Chlorella sp. showed antibacterial activity against B. subtilis ATCC 6633 (625 µg/ml and 1250 µg/ml, respectively), E. faecalis ATCC 29212 (>5000 µg/ml and 1250 µg/ml, respectively), E. coli ATCC 25922 (>5000 µg/ml), P. aeruginosa ATCC 27853 (>5000 µg/ml), S. aureus ATCC 29213 (2500 µg/ml) at the specified concentrations. In the chemical analysis of the extracts, it was determined that the fatty acids were in high amounts, 33.22% and 40.41%, respectively, in the methanol and acetone extracts. Among the alternative methods to show activity against pathogenic microorganisms, algae can be a good natural resource. This study showed that Chlorella sp. contains high fatty acids and has potential as an antibacterial agent of natural origin.</jats:p>},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
<jats:p xml:lang="en">In this study, the antibacterial activity of methanol and acetone extracts of Chlorella sp. was examined. The chemical contents of the extracts were clarified by GC/MS analysis. Antibacterial activity of Chlorella sp. extracts was determined as a minimum inhibitory concentration by broth microdilution method against Bacillus subtilis ATCC 6633, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 29213. It was found that methanol and acetone extracts of Chlorella sp. showed antibacterial activity against B. subtilis ATCC 6633 (625 µg/ml and 1250 µg/ml, respectively), E. faecalis ATCC 29212 (>5000 µg/ml and 1250 µg/ml, respectively), E. coli ATCC 25922 (>5000 µg/ml), P. aeruginosa ATCC 27853 (>5000 µg/ml), S. aureus ATCC 29213 (2500 µg/ml) at the specified concentrations. In the chemical analysis of the extracts, it was determined that the fatty acids were in high amounts, 33.22% and 40.41%, respectively, in the methanol and acetone extracts. Among the alternative methods to show activity against pathogenic microorganisms, algae can be a good natural resource. This study showed that Chlorella sp. contains high fatty acids and has potential as an antibacterial agent of natural origin.</jats:p>
Kiymaci, Merve Eylul; Simsek, Duygu; Tok, Kenan Can; Dirican, Dilay; Gumustas, Mehmet
Probiotic Potential and Anti-quorum Sensing Activity of Enterococcus faecalis and Lactobacillus acidophilus Isolates from Apis mellifera Journal Article
In: Journal of the Hellenic Veterinary Medical Society, vol. 73, no. 4, pp. 4717-4728, 2023, ISSN: 2585-3724.
@article{Kiymaci2022b,
title = {Probiotic Potential and Anti-quorum Sensing Activity of Enterococcus faecalis and Lactobacillus acidophilus Isolates from Apis mellifera},
author = {Merve Eylul Kiymaci and Duygu Simsek and Kenan Can Tok and Dilay Dirican and Mehmet Gumustas},
doi = {10.12681/jhvms.26743},
issn = {2585-3724},
year = {2023},
date = {2023-01-21},
urldate = {2023-01-21},
journal = {Journal of the Hellenic Veterinary Medical Society},
volume = {73},
number = {4},
pages = {4717-4728},
abstract = {One health is an approach that gains importance in the protection of public health and advocates that the environment-animal-human health is linked. Microbial resistance occurring in any of these three factors circulates directly or indirectly between species and affects health. In this study, antimicrobial, anti-quorum sensing activity and potential probiotic properties of Lactobacillus acidophilus and Enterococcus faecalis 1 and 2 isolates from Apis mellifera gut were investigated. Results showed that isolates were found to be resistant to gentamicin, streptomycin, erythromycin, and clindamycin tested. It was determined that isolates were gamma haemolytic and resistant to acid and enzymatic environment conditions. Enterococcus faecalis isolates had antimicrobial activity on Escherichia coli ATCC 25922, Enterococcus faecalis ATCC 29212 and Pseudomonas aeruginosa ATCC 27853 and anti-quorum sensing activity of the culture supernatants of all isolates was detected by a violacein pigment inhibition of Chromobacterium violaceum strain.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
One health is an approach that gains importance in the protection of public health and advocates that the environment-animal-human health is linked. Microbial resistance occurring in any of these three factors circulates directly or indirectly between species and affects health. In this study, antimicrobial, anti-quorum sensing activity and potential probiotic properties of Lactobacillus acidophilus and Enterococcus faecalis 1 and 2 isolates from Apis mellifera gut were investigated. Results showed that isolates were found to be resistant to gentamicin, streptomycin, erythromycin, and clindamycin tested. It was determined that isolates were gamma haemolytic and resistant to acid and enzymatic environment conditions. Enterococcus faecalis isolates had antimicrobial activity on Escherichia coli ATCC 25922, Enterococcus faecalis ATCC 29212 and Pseudomonas aeruginosa ATCC 27853 and anti-quorum sensing activity of the culture supernatants of all isolates was detected by a violacein pigment inhibition of Chromobacterium violaceum strain.
2022
Tok, Kenan Can; Hurkul, Muhammed Mesud; Bozkurt, Nazmiye Neslihan; Aysal, Ayhan Ibrahim; Yayla, Seyda
Investigation of Phytochemicals in Methanolic Herba Extract of Endemic Silene ruscifolia by LC-QTOF/MS and GC/MS Journal Article
In: Journal of Faculty of Pharmacy of Ankara University, vol. 46, no. 3, pp. 827-838, 2022, ISSN: 2564-6524.
@article{Tok2023,
title = {Investigation of Phytochemicals in Methanolic Herba Extract of Endemic Silene ruscifolia by LC-QTOF/MS and GC/MS},
author = {Kenan Can Tok and Muhammed Mesud Hurkul and Nazmiye Neslihan Bozkurt and Ayhan Ibrahim Aysal and Seyda Yayla},
doi = {Doi: 10.33483/jfpau.1133615},
issn = {2564-6524},
year = {2022},
date = {2022-09-30},
urldate = {2022-09-30},
journal = {Journal of Faculty of Pharmacy of Ankara University},
volume = {46},
number = {3},
pages = {827-838},
abstract = {Objective: Silene L. (Caryophyllaceae) species are traditionally used for the treatment of inflammation, urinary inflammation, eye trouble, skin problem, stomachache, dysentery, tooth decay, fever, headache, malaria, pimples, and backache. Chemical ingredients of Silene species consist of flavonoids, anthocyanidins, terpenoids, triterpene saponins, phytoecdysteroids, benzenoids, vitamins, and show antioxidants, anti-inflammatory, antitumor, and antiviral activities. Silene ruscifolia (Hub.-Mor. & Reese) Hub.-Mor. are endemic to Türkiye and called as "gizli nakıl".
Material and Method: The plant material was collected from Beynam Forest (Ankara/Türkiye). The aerial parts of the plant were extracted with methanol in an ultrasonic bath. HPLC system (Agilent 1260 Series) with an autosampler, binary pump, column oven, and a UV detector was coupled with an iFunnel Quadrupole Time-of-Flight LC-MS system (Agilent G6550A) with a Dual Spray Agilent Jet Stream electrospray ionization source. Agilent TC C-18 (4.6 mm x 150 mm x 5 µm) column was used for the separation of the compounds. The GC-MS analysis of extract was performed using an Agilent 6890 gas chromatograph equipped with an Agilent 5973N quadrupole mass spectrometer (Agilent, USA). Mass Hunter software (Qualitative Analysis B.07.00) and the NIST Mass Spectral Library (2014) were used to determine and identify the compounds.
Result and Discussion: LC-MS Q-TOF analysis showed that S. ruscifolia contained rutin, narcissin, luteolin, isorhamnetin, rhamnetin, and quercetin dimethyl ether. GC-MS analysis showed that the extract had the highest content of sugar (50.5%) and sugar alcohols (46.39%). It also contains carboxylic acid (0.47%), fatty acid (0.64%), sugar acid (0.42%), glycoside (0.48%), carotenoids (0.61%) and benzoic acid ester (0.49%). D-pinitol has the highest content in the extract with 41.14%.
},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Objective: Silene L. (Caryophyllaceae) species are traditionally used for the treatment of inflammation, urinary inflammation, eye trouble, skin problem, stomachache, dysentery, tooth decay, fever, headache, malaria, pimples, and backache. Chemical ingredients of Silene species consist of flavonoids, anthocyanidins, terpenoids, triterpene saponins, phytoecdysteroids, benzenoids, vitamins, and show antioxidants, anti-inflammatory, antitumor, and antiviral activities. Silene ruscifolia (Hub.-Mor. & Reese) Hub.-Mor. are endemic to Türkiye and called as "gizli nakıl".
Material and Method: The plant material was collected from Beynam Forest (Ankara/Türkiye). The aerial parts of the plant were extracted with methanol in an ultrasonic bath. HPLC system (Agilent 1260 Series) with an autosampler, binary pump, column oven, and a UV detector was coupled with an iFunnel Quadrupole Time-of-Flight LC-MS system (Agilent G6550A) with a Dual Spray Agilent Jet Stream electrospray ionization source. Agilent TC C-18 (4.6 mm x 150 mm x 5 µm) column was used for the separation of the compounds. The GC-MS analysis of extract was performed using an Agilent 6890 gas chromatograph equipped with an Agilent 5973N quadrupole mass spectrometer (Agilent, USA). Mass Hunter software (Qualitative Analysis B.07.00) and the NIST Mass Spectral Library (2014) were used to determine and identify the compounds.
Result and Discussion: LC-MS Q-TOF analysis showed that S. ruscifolia contained rutin, narcissin, luteolin, isorhamnetin, rhamnetin, and quercetin dimethyl ether. GC-MS analysis showed that the extract had the highest content of sugar (50.5%) and sugar alcohols (46.39%). It also contains carboxylic acid (0.47%), fatty acid (0.64%), sugar acid (0.42%), glycoside (0.48%), carotenoids (0.61%) and benzoic acid ester (0.49%). D-pinitol has the highest content in the extract with 41.14%.
Material and Method: The plant material was collected from Beynam Forest (Ankara/Türkiye). The aerial parts of the plant were extracted with methanol in an ultrasonic bath. HPLC system (Agilent 1260 Series) with an autosampler, binary pump, column oven, and a UV detector was coupled with an iFunnel Quadrupole Time-of-Flight LC-MS system (Agilent G6550A) with a Dual Spray Agilent Jet Stream electrospray ionization source. Agilent TC C-18 (4.6 mm x 150 mm x 5 µm) column was used for the separation of the compounds. The GC-MS analysis of extract was performed using an Agilent 6890 gas chromatograph equipped with an Agilent 5973N quadrupole mass spectrometer (Agilent, USA). Mass Hunter software (Qualitative Analysis B.07.00) and the NIST Mass Spectral Library (2014) were used to determine and identify the compounds.
Result and Discussion: LC-MS Q-TOF analysis showed that S. ruscifolia contained rutin, narcissin, luteolin, isorhamnetin, rhamnetin, and quercetin dimethyl ether. GC-MS analysis showed that the extract had the highest content of sugar (50.5%) and sugar alcohols (46.39%). It also contains carboxylic acid (0.47%), fatty acid (0.64%), sugar acid (0.42%), glycoside (0.48%), carotenoids (0.61%) and benzoic acid ester (0.49%). D-pinitol has the highest content in the extract with 41.14%.
Tok, Kenan Can; Gumustas, Mehmet; Sengel-Turk, Ceyda Tuba; Amasya, Gulin; Bayram, Bilge; Arioglu-Inan, Ebru
Development of salting-out extraction methodology for the determination of piroxicam from polymeric based nanocarriers and biological samples Journal Article
In: J Pharm Biomed Anal, vol. 219, pp. 114966, 2022, ISSN: 1873-264X.
@article{pmid35908414,
title = {Development of salting-out extraction methodology for the determination of piroxicam from polymeric based nanocarriers and biological samples},
author = {Kenan Can Tok and Mehmet Gumustas and Ceyda Tuba Sengel-Turk and Gulin Amasya and Bilge Bayram and Ebru Arioglu-Inan},
doi = {10.1016/j.jpba.2022.114966},
issn = {1873-264X},
year = {2022},
date = {2022-09-01},
urldate = {2022-09-01},
journal = {J Pharm Biomed Anal},
volume = {219},
pages = {114966},
abstract = {The aim of the present study is to develop the polymeric nanoparticulate drug delivery systems of piroxicam and to evaluate the in-vitro characteristics such as entrapment efficiency, surface morphology, in-vitro drug release performance, etc. For this reason, a novel HPLC methodology was developed for the determination of piroxicam from its bulk form, pharmaceutical preparation, and nanoparticulate delivery systems. Furthermore, the developed formulation was applied to the rats and the biological samples (plasma, liver, heart, spleen, kidney, and lung homogenates) were analyzed by the developed HPLC method following a salting-out assisted liquid-liquid extraction strategy for the first time in the literature. A Kinetex C18 analytical column (150 mm × 4.6 mm i.d., 5 µm) was used as a stationary phase with a 0.8 mL/min flow rate of acetonitrile: phosphate buffer (40:60, v/v), the column oven was adjusted to 40 °C and detection wavelength is set to 360 nm. Developed method were validated as per selectivity, linearity, LOD, LOQ, precision, and accuracy specified in the International Council for Harmonisation guidelines. As a result of the present study, it has been shown that the analysis of piroxicam from the bulk form, pharmaceutical preparation, developed polymeric-based drug delivery system, and biological samples can be successfully performed and no interferences were observed in any matrix. The developed method was also successfully utilized to study the tissue distribution of piroxicam in rats.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
The aim of the present study is to develop the polymeric nanoparticulate drug delivery systems of piroxicam and to evaluate the in-vitro characteristics such as entrapment efficiency, surface morphology, in-vitro drug release performance, etc. For this reason, a novel HPLC methodology was developed for the determination of piroxicam from its bulk form, pharmaceutical preparation, and nanoparticulate delivery systems. Furthermore, the developed formulation was applied to the rats and the biological samples (plasma, liver, heart, spleen, kidney, and lung homogenates) were analyzed by the developed HPLC method following a salting-out assisted liquid-liquid extraction strategy for the first time in the literature. A Kinetex C18 analytical column (150 mm × 4.6 mm i.d., 5 µm) was used as a stationary phase with a 0.8 mL/min flow rate of acetonitrile: phosphate buffer (40:60, v/v), the column oven was adjusted to 40 °C and detection wavelength is set to 360 nm. Developed method were validated as per selectivity, linearity, LOD, LOQ, precision, and accuracy specified in the International Council for Harmonisation guidelines. As a result of the present study, it has been shown that the analysis of piroxicam from the bulk form, pharmaceutical preparation, developed polymeric-based drug delivery system, and biological samples can be successfully performed and no interferences were observed in any matrix. The developed method was also successfully utilized to study the tissue distribution of piroxicam in rats.
Simsek, Duygu; Kiymaci, Merve Eylul; Tok, Kenan Can; Gumustas, Mehmet; Altanlar, Nurten
Investigation of the Probiotic and Metabolic Potential of Fructobacillus tropaeoli and Apilactobacillus kunkeei from Apiaries Journal Article
In: Archives of Microbiology, vol. 204, no. 7, pp. 432, 2022, ISSN: 0302-8933.
@article{Simsek2022,
title = {Investigation of the Probiotic and Metabolic Potential of Fructobacillus tropaeoli and Apilactobacillus kunkeei from Apiaries},
author = {Duygu Simsek and Merve Eylul Kiymaci and Kenan Can Tok and Mehmet Gumustas and Nurten Altanlar},
url = {https://link.springer.com/article/10.1007/s00203-022-03000-x},
doi = {10.1007/s00203-022-03000-x},
issn = {0302-8933},
year = {2022},
date = {2022-06-27},
urldate = {2022-06-27},
journal = {Archives of Microbiology},
volume = {204},
number = {7},
pages = {432},
abstract = {Honeybee products have been among important consumer products throughout history. Microbiota has attracted attention in recent years due to both their probiotic value and industrial potential. Fructophilic lactic acid bacteria (FLAB), whose field of study has been expanding rapidly in the last 20 years, are among the groups that can be isolated from the bee gut. This study aimed to isolate FLAB from the honeybees of two different geographic regions in Turkey and investigate their probiotic, metabolic and anti-quorum sensing (anti-QS) potential. Metabolic properties were investigated based on fructose toleration and acid and diacetyl production while the probiotic properties of the isolates were determined by examining pH, pepsin, pancreatin resistance, antimicrobial susceptibility, and antimicrobial activity. Anti-QS activities were also evaluated with the Chromobacterium violaceum biosensor strain. Two FLAB members were isolated and identified by the 16S rRNA analysis as Fructobacillus tropaeoli and Apilactobacillus kunkeei, which were found to be tolerant to high fructose, low pH, pepsin, pancreatin, and bile salt environments. Both isolates showed anti-QS activity against the C. violaceum biosensor strain and no diacetyl production. The daily supernatants of the isolates inhibited the growth of Enterococcus faecalis ATCC 29212 among the selected pathogens. The isolates were found resistant to kanamycin, streptomycin, erythromycin, and clindamycin. In the evaluation of the probiotic potential of these species, the negative effect of antibiotics and other chemicals to which honeybees are directly or indirectly exposed draws attention within the scope of the “One Health” approach.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Honeybee products have been among important consumer products throughout history. Microbiota has attracted attention in recent years due to both their probiotic value and industrial potential. Fructophilic lactic acid bacteria (FLAB), whose field of study has been expanding rapidly in the last 20 years, are among the groups that can be isolated from the bee gut. This study aimed to isolate FLAB from the honeybees of two different geographic regions in Turkey and investigate their probiotic, metabolic and anti-quorum sensing (anti-QS) potential. Metabolic properties were investigated based on fructose toleration and acid and diacetyl production while the probiotic properties of the isolates were determined by examining pH, pepsin, pancreatin resistance, antimicrobial susceptibility, and antimicrobial activity. Anti-QS activities were also evaluated with the Chromobacterium violaceum biosensor strain. Two FLAB members were isolated and identified by the 16S rRNA analysis as Fructobacillus tropaeoli and Apilactobacillus kunkeei, which were found to be tolerant to high fructose, low pH, pepsin, pancreatin, and bile salt environments. Both isolates showed anti-QS activity against the C. violaceum biosensor strain and no diacetyl production. The daily supernatants of the isolates inhibited the growth of Enterococcus faecalis ATCC 29212 among the selected pathogens. The isolates were found resistant to kanamycin, streptomycin, erythromycin, and clindamycin. In the evaluation of the probiotic potential of these species, the negative effect of antibiotics and other chemicals to which honeybees are directly or indirectly exposed draws attention within the scope of the “One Health” approach.
Tok, Kenan Can; Yayla, Seyda
Gas Chromatography-Mass Spectrometry (GC-MS) Analysis of Consolida thirkeana Extract Journal Article
In: Journal of Faculty of Pharmacy of Ankara University, vol. 46, no. 2, pp. 444-449, 2022.
@article{Tok2022,
title = {Gas Chromatography-Mass Spectrometry (GC-MS) Analysis of Consolida thirkeana Extract},
author = {Kenan Can Tok and Seyda Yayla},
doi = {10.33483/jfpau.1102380},
year = {2022},
date = {2022-05-31},
urldate = {2022-05-31},
journal = {Journal of Faculty of Pharmacy of Ankara University},
volume = {46},
number = {2},
pages = {444-449},
abstract = {Objective: Consolida thirkeana (Boiss.) Bornm. (Ranunculaceae) is considered endemic to Turkey and identified by pale lilac flowers, sessile follicles and laciniae linear leaves. The genus Consolida can often be confused morphologically with Delphinium, in this respect, members of the genus Consolida need chemotaxonomic interest. In this study, the phytochemical content of C. thirkeana was clarified by GC/MS analysis.
Material and Method: The plant material was collected from Ayaş (Ankara/Turkey). A voucher specimen was deposited in the Ankara University Faculty of Pharmacy Herbarium. The aerial part of the plant was powdered in a grinder. The powdered plant parts were macerated with methanol. The GC-MS analysis of extracts were performed using an Agilent 6890 gas chromatograph equipped with an Agilent 5973N quadrupole mass spectrometer (Agilent, USA). Mass Hunter software (Qualitative Analysis B.07.00) and the NIST Mass Spectral Library (2014) were used for determining and identifying compounds.
Result and Discussion: The analysis results of this study showed that, the presence of 15 compounds in C. thirkeana methanol extract. The extract contains 0.65% Glycoside, 0.68% Fatty acid, 0.91% Acylglycerol, 2.41% Carboxylic acid, 38.5% Sugar, 56.86% Sugar alcohol.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Objective: Consolida thirkeana (Boiss.) Bornm. (Ranunculaceae) is considered endemic to Turkey and identified by pale lilac flowers, sessile follicles and laciniae linear leaves. The genus Consolida can often be confused morphologically with Delphinium, in this respect, members of the genus Consolida need chemotaxonomic interest. In this study, the phytochemical content of C. thirkeana was clarified by GC/MS analysis.
Material and Method: The plant material was collected from Ayaş (Ankara/Turkey). A voucher specimen was deposited in the Ankara University Faculty of Pharmacy Herbarium. The aerial part of the plant was powdered in a grinder. The powdered plant parts were macerated with methanol. The GC-MS analysis of extracts were performed using an Agilent 6890 gas chromatograph equipped with an Agilent 5973N quadrupole mass spectrometer (Agilent, USA). Mass Hunter software (Qualitative Analysis B.07.00) and the NIST Mass Spectral Library (2014) were used for determining and identifying compounds.
Result and Discussion: The analysis results of this study showed that, the presence of 15 compounds in C. thirkeana methanol extract. The extract contains 0.65% Glycoside, 0.68% Fatty acid, 0.91% Acylglycerol, 2.41% Carboxylic acid, 38.5% Sugar, 56.86% Sugar alcohol.
Material and Method: The plant material was collected from Ayaş (Ankara/Turkey). A voucher specimen was deposited in the Ankara University Faculty of Pharmacy Herbarium. The aerial part of the plant was powdered in a grinder. The powdered plant parts were macerated with methanol. The GC-MS analysis of extracts were performed using an Agilent 6890 gas chromatograph equipped with an Agilent 5973N quadrupole mass spectrometer (Agilent, USA). Mass Hunter software (Qualitative Analysis B.07.00) and the NIST Mass Spectral Library (2014) were used for determining and identifying compounds.
Result and Discussion: The analysis results of this study showed that, the presence of 15 compounds in C. thirkeana methanol extract. The extract contains 0.65% Glycoside, 0.68% Fatty acid, 0.91% Acylglycerol, 2.41% Carboxylic acid, 38.5% Sugar, 56.86% Sugar alcohol.
Kiymaci, Merve Eylül; Tok, Kenan Can; Hurkul, Muhammed Mesud
In: Journal of Faculty of Pharmacy of Ankara University, vol. 46, no. 1, pp. 160 - 169, 2022, ISSN: 1015-3918.
@article{Kiymaci2022,
title = {A Study on Phytochemical Analysis and Antibacterial Activity of Querqus Macranthera Subsp. Syspirensis (K.Koch) Menitsky Branch and Leaf Extracts},
author = {Merve Eylül Kiymaci and Kenan Can Tok and Muhammed Mesud Hurkul},
url = {https://dergipark.org.tr/tr/pub/jfpanu/issue/68300/1034549},
doi = {10.33483/jfpau.1034549},
issn = {1015-3918},
year = {2022},
date = {2022-01-29},
urldate = {2022-01-29},
journal = {Journal of Faculty of Pharmacy of Ankara University},
volume = {46},
number = {1},
pages = {160 - 169},
publisher = {Ankara Üniversitesi},
address = {Ankara University Faculty of Pharmacy Degol Str. TR-06100 Tandogan/Ankra/TURKEY},
abstract = {Objective: Oak species are medicinal plants with traditional use around the world. These species, which are very rich in tannins, have potential as antibacterial agents in terms of the polyphenolic compounds content. In this study, the antibacterial potential and phytochemical content of the branches and leaves of Quercus macranthera subsp. syspirensis, which is endemic to Turkey, were investigated.
Material and Method: Plant materials were collected from Araç (Kastamonu/Turkey) in 2020. Methanol extracts were prepared from dried and powdered branches and leaves. The antibacterial activity test was evaluated by broth microdilution method as a minimal inhibition concentration (MIC) against Staphylococcus aureus ATCC 29213, Staphylococcus epidermidis ATCC 35984, Enterococcus faecalis ATCC 29212, Klebsiella pneumoniae ATCC 13883, Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, Acinetobacter baumannii ATCC 19606 and Bacillus subtilis ATCC 6633. The GC-MS analysis of extracts were performed using an Agilent 6890 gas chromatograph equipped with an Agilent 5973N quadrupole mass spectrometer (Agilent, USA). The compounds were identified by comparing the mass spectrum ratio of the sample with the data available in NIST 2014 Mass Spectral Library.
Result and Discussion: As a result, it was found that the branch extracts were more effective than the leaf extracts and both branch and leaf extracts showed the highest activity against Bacillus subtilis ATCC 6633 strain (48.8 µg/ml, 97.6 µg/ml, respectively). The extracts also showed antibacterial activity at varying concentrations on other test strains.},
key = {Antibacterial, branch, GC-MS, leaf, Quercus macranthera subsp. syspirensis},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Objective: Oak species are medicinal plants with traditional use around the world. These species, which are very rich in tannins, have potential as antibacterial agents in terms of the polyphenolic compounds content. In this study, the antibacterial potential and phytochemical content of the branches and leaves of Quercus macranthera subsp. syspirensis, which is endemic to Turkey, were investigated.
Material and Method: Plant materials were collected from Araç (Kastamonu/Turkey) in 2020. Methanol extracts were prepared from dried and powdered branches and leaves. The antibacterial activity test was evaluated by broth microdilution method as a minimal inhibition concentration (MIC) against Staphylococcus aureus ATCC 29213, Staphylococcus epidermidis ATCC 35984, Enterococcus faecalis ATCC 29212, Klebsiella pneumoniae ATCC 13883, Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, Acinetobacter baumannii ATCC 19606 and Bacillus subtilis ATCC 6633. The GC-MS analysis of extracts were performed using an Agilent 6890 gas chromatograph equipped with an Agilent 5973N quadrupole mass spectrometer (Agilent, USA). The compounds were identified by comparing the mass spectrum ratio of the sample with the data available in NIST 2014 Mass Spectral Library.
Result and Discussion: As a result, it was found that the branch extracts were more effective than the leaf extracts and both branch and leaf extracts showed the highest activity against Bacillus subtilis ATCC 6633 strain (48.8 µg/ml, 97.6 µg/ml, respectively). The extracts also showed antibacterial activity at varying concentrations on other test strains.
Material and Method: Plant materials were collected from Araç (Kastamonu/Turkey) in 2020. Methanol extracts were prepared from dried and powdered branches and leaves. The antibacterial activity test was evaluated by broth microdilution method as a minimal inhibition concentration (MIC) against Staphylococcus aureus ATCC 29213, Staphylococcus epidermidis ATCC 35984, Enterococcus faecalis ATCC 29212, Klebsiella pneumoniae ATCC 13883, Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, Acinetobacter baumannii ATCC 19606 and Bacillus subtilis ATCC 6633. The GC-MS analysis of extracts were performed using an Agilent 6890 gas chromatograph equipped with an Agilent 5973N quadrupole mass spectrometer (Agilent, USA). The compounds were identified by comparing the mass spectrum ratio of the sample with the data available in NIST 2014 Mass Spectral Library.
Result and Discussion: As a result, it was found that the branch extracts were more effective than the leaf extracts and both branch and leaf extracts showed the highest activity against Bacillus subtilis ATCC 6633 strain (48.8 µg/ml, 97.6 µg/ml, respectively). The extracts also showed antibacterial activity at varying concentrations on other test strains.
2021
Karadeniz, Demet Genc; Kaskatepe, Banu; Kiymaci, Merve Eylul; Tok, Kenan Can; Gumustas, Mehmet; Karaaslan, Cigdem
Microbial Exopolysaccharide Production of Streptococcus thermophilus and Its Antiquorum Sensing Activity Journal Article
In: Archives of Microbiology, vol. 203, no. 6, pp. 3331–3339, 2021, ISSN: 0302-8933.
@article{Karadeniz2021,
title = {Microbial Exopolysaccharide Production of Streptococcus thermophilus and Its Antiquorum Sensing Activity},
author = {Demet Genc Karadeniz and Banu Kaskatepe and Merve Eylul Kiymaci and Kenan Can Tok and Mehmet Gumustas and Cigdem Karaaslan},
url = {https://pubmed.ncbi.nlm.nih.gov/33866380/
https://link.springer.com/article/10.1007%2Fs00203-021-02313-7},
doi = {10.1007/s00203-021-02313-7},
issn = {0302-8933},
year = {2021},
date = {2021-04-18},
urldate = {2021-04-18},
journal = {Archives of Microbiology},
volume = {203},
number = {6},
pages = {3331–3339},
abstract = {Interest in the production of exopolysaccharides by microorganisms has increased in the recent years. Using low-cost product is the main step of microbial production to reduce cost and compete with chemical production. In this work, EPS production of Streptococcus thermophilus isolates from yogurt (S2), kefir (S3), and S. thermophilus ATCC 19258 (S1) isolate which was used as control strains were investigated by using different fruit pulps. S. thermophilus isolates were identified by morphological and 16S sequence analysis. The amount of EPS obtained was measured spectrophotometrically using glucose as standard with phenol sulfuric acid method. All three isolates produced higher amounts of EPS on M17 medium than Nutrient medium. When the fruit pulp was added to the medium, EPS production increased in all three isolates. When different nitrogen sources were added together with fruit pulp juice, EPS production increased. The highest amount of EPS produced by ATCC 19258 strain (21.570 mg/L) and S3 isolate (29.131 mg/L) is the medium where mixed fruit pulp juice and nitrogen source is tryptophan. It has been shown that EPS production is increased by adding fruit pulps to the prepared media. It is thought that apricot pulp can be a good alternative in EPS production especially in the evaluation of wastes. Also, antiquorum sensing activity of the highest amount EPS was determined by using Chromobacterium violaceum CV026 strain and found effective on violacein pigment inhibition and C6-AHL production of biosensor strain.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Interest in the production of exopolysaccharides by microorganisms has increased in the recent years. Using low-cost product is the main step of microbial production to reduce cost and compete with chemical production. In this work, EPS production of Streptococcus thermophilus isolates from yogurt (S2), kefir (S3), and S. thermophilus ATCC 19258 (S1) isolate which was used as control strains were investigated by using different fruit pulps. S. thermophilus isolates were identified by morphological and 16S sequence analysis. The amount of EPS obtained was measured spectrophotometrically using glucose as standard with phenol sulfuric acid method. All three isolates produced higher amounts of EPS on M17 medium than Nutrient medium. When the fruit pulp was added to the medium, EPS production increased in all three isolates. When different nitrogen sources were added together with fruit pulp juice, EPS production increased. The highest amount of EPS produced by ATCC 19258 strain (21.570 mg/L) and S3 isolate (29.131 mg/L) is the medium where mixed fruit pulp juice and nitrogen source is tryptophan. It has been shown that EPS production is increased by adding fruit pulps to the prepared media. It is thought that apricot pulp can be a good alternative in EPS production especially in the evaluation of wastes. Also, antiquorum sensing activity of the highest amount EPS was determined by using Chromobacterium violaceum CV026 strain and found effective on violacein pigment inhibition and C6-AHL production of biosensor strain.
2020
Ozdemir, Fezile; Kir, Yagmur; Tok, Kenan Can; Baskak, Bora; Suzen, Halit Sinan
In: Turkish Journal of Pharmaceutical Science, vol. 17, no. 6, pp. 653–658, 2020, ISSN: 2148-6247.
@article{Ozdemir2020,
title = {A Novel Genotyping Method for Detection of the Muscarinic Receptor M1 Gene rs2067477 Polymorphism and Its Genotype/Allele Frequencies in a Turkish Population},
author = {Fezile Ozdemir and Yagmur Kir and Kenan Can Tok and Bora Baskak and Halit Sinan Suzen},
url = {https://pubmed.ncbi.nlm.nih.gov/33389966/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7786075/
https://turkjps.org/archives/archive-detail/article-preview/a-novel-genotyping-method-for-detection-of-the-mus/32884
https://app.trdizin.gov.tr/makale/TkRBM05EUTBOQT09/a-novel-genotyping-method-for-detection-of-the-muscarinic-receptor-m1-gene-rs2067477-polymorphism-and-its-genotype-allele-frequencies-in-a-turkish-population},
doi = {10.4274/tjps.galenos.2019.46793},
issn = {2148-6247},
year = {2020},
date = {2020-12-23},
urldate = {2020-12-23},
journal = {Turkish Journal of Pharmaceutical Science},
volume = {17},
number = {6},
pages = {653–658},
abstract = {Objectives:
Gene variation in the cholinergic muscarinic receptor 1 (CHRM1) has potential to become a candidate biomarker in the development of several disorders as well as drug response. In this study, a novel polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay was developed to determine the C to A single nucleotide polymorphism at position 267 in the CHRM1 gene.
Materials and Methods:
A new reverse primer and a mismatched forward primer were designed to obtain 125 bp PCR products. The PCR products were then digested with the Hae III restriction enzyme to detect the rs2067477 polymorphism that comprises a C to A base change. The novel assay developed was tested in 51 Turkish schizophrenia patients.
Results:
The genotyping assay was successfully performed in patients with schizophrenia in order to confirm the accuracy and validity of this method. The frequency of CC, CA, and AA genotypes was 72.5%, 25.5%, and 2%, respectively. On the basis of these findings, the allele frequency of C was 0.85 and the allele frequency of A was 0.15.
Conclusion:
This genotyping assay is practical for screening the CHRM1 C267A polymorphism in pharmacogenetic studies. The present polymorphism may be used as a candidate biomarker to determine genetic susceptibility to related diseases and may contribute to the implementation of individualized drug therapy for M1-related diseases.},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
Objectives:
Gene variation in the cholinergic muscarinic receptor 1 (CHRM1) has potential to become a candidate biomarker in the development of several disorders as well as drug response. In this study, a novel polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay was developed to determine the C to A single nucleotide polymorphism at position 267 in the CHRM1 gene.
Materials and Methods:
A new reverse primer and a mismatched forward primer were designed to obtain 125 bp PCR products. The PCR products were then digested with the Hae III restriction enzyme to detect the rs2067477 polymorphism that comprises a C to A base change. The novel assay developed was tested in 51 Turkish schizophrenia patients.
Results:
The genotyping assay was successfully performed in patients with schizophrenia in order to confirm the accuracy and validity of this method. The frequency of CC, CA, and AA genotypes was 72.5%, 25.5%, and 2%, respectively. On the basis of these findings, the allele frequency of C was 0.85 and the allele frequency of A was 0.15.
Conclusion:
This genotyping assay is practical for screening the CHRM1 C267A polymorphism in pharmacogenetic studies. The present polymorphism may be used as a candidate biomarker to determine genetic susceptibility to related diseases and may contribute to the implementation of individualized drug therapy for M1-related diseases.
Gene variation in the cholinergic muscarinic receptor 1 (CHRM1) has potential to become a candidate biomarker in the development of several disorders as well as drug response. In this study, a novel polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay was developed to determine the C to A single nucleotide polymorphism at position 267 in the CHRM1 gene.
Materials and Methods:
A new reverse primer and a mismatched forward primer were designed to obtain 125 bp PCR products. The PCR products were then digested with the Hae III restriction enzyme to detect the rs2067477 polymorphism that comprises a C to A base change. The novel assay developed was tested in 51 Turkish schizophrenia patients.
Results:
The genotyping assay was successfully performed in patients with schizophrenia in order to confirm the accuracy and validity of this method. The frequency of CC, CA, and AA genotypes was 72.5%, 25.5%, and 2%, respectively. On the basis of these findings, the allele frequency of C was 0.85 and the allele frequency of A was 0.15.
Conclusion:
This genotyping assay is practical for screening the CHRM1 C267A polymorphism in pharmacogenetic studies. The present polymorphism may be used as a candidate biomarker to determine genetic susceptibility to related diseases and may contribute to the implementation of individualized drug therapy for M1-related diseases.
Tok, Kenan Can; Gumustas, Mehmet; Jibuti, Giorgi; Suzen, Halit Sinan; Ozkan, Sibel A.; Chankvetadze, Bezhan
In: Molecules, vol. 25, no. 24, pp. 5865, 2020, ISSN: 1420-3049.
@article{Tok2020,
title = {The Effect of Enantiomer Elution Order on the Determination of Minor Enantiomeric Impurity in Ketoprofen and Enantiomeric Purity Evaluation of Commercially Available Dexketoprofen Formulations},
author = {Kenan Can Tok and Mehmet Gumustas and Giorgi Jibuti and Halit Sinan Suzen and Sibel A. Ozkan and Bezhan Chankvetadze},
url = {https://pubmed.ncbi.nlm.nih.gov/33322449/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7763306/
https://www.mdpi.com/1420-3049/25/24/5865},
doi = {10.3390/molecules25245865},
issn = {1420-3049},
year = {2020},
date = {2020-12-11},
urldate = {2020-12-11},
journal = {Molecules},
volume = {25},
number = {24},
pages = {5865},
abstract = {In a recent study, opposite enantiomer elution order was observed for ketoprofen enantiomers on two amylose-phenylcarbamate-based chiral columns with the same chemical composition of the chiral selector but in one case with coated while in the other with an immobilized chiral selector. In the present study, the influence of this uncommon effect on method validation parameters for the determination of minor enantiomeric impurity in dexketoprofen was studied. The validated methods with two alternative elution orders for enantiomers were applied for the evaluation of enantiomeric impurity in six marketed dexketoprofen formulations from various vendors. In most of these formulations except one the content of enantiomeric impurity exceeded 0.1% (w/w).},
keywords = {},
pubstate = {published},
tppubtype = {article}
}
In a recent study, opposite enantiomer elution order was observed for ketoprofen enantiomers on two amylose-phenylcarbamate-based chiral columns with the same chemical composition of the chiral selector but in one case with coated while in the other with an immobilized chiral selector. In the present study, the influence of this uncommon effect on method validation parameters for the determination of minor enantiomeric impurity in dexketoprofen was studied. The validated methods with two alternative elution orders for enantiomers were applied for the evaluation of enantiomeric impurity in six marketed dexketoprofen formulations from various vendors. In most of these formulations except one the content of enantiomeric impurity exceeded 0.1% (w/w).